Putative R-Genes, predicted from NCBI UniGene
UniGene is an NCBI database organising GenBank sequences into a non-redundant set of gene-oriented clusters[1].
UniGene computationally identifies transcripts from the same locus providing sets of transcript sequences that appear to come from the same transcription locus (both genes and expressed pseudogenes).
Information about selected protein similarities (comparison of cluster transcripts with RefSeq proteins), gene expression (tissues and development stages from specific gene's sequences), mapping position (genomic location) and sequences (mRNAs, ESTs, and gene predictions supported by transcribed sequences) [2].
Novel putative R-genes have been computationally predicted from the UniGene dataset, using a bioinformatic pipeline, Disease Resistance Analysis and Gene Orthology (DRAGO pipeline)
[1] Wheeler DL, Chappey C, Lash AE, Leipe DD, Madden TL, Schuler GD, Tatusova TA, Rapp BA. Database resources of the National Center for Biotechnology Information. Nucleic Acids Res. 2000 Jan 1;28(1):10-4.
PMID:10592169[1]
